Glycoengineering of antibody (Herceptin) through yeast expression and in vitro enzymatic glycosylation
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چکیده
منابع مشابه
Expression, purification and glycosylation analysis of chicken infectious bursal disease virus VP2 in yeast
Infectious bursal disease (IBD), a highly contagious and devastating disease in young chicken, is causedby infectious bursal disease virus (IBDV). To improve the immunogenicity of recombinant IBDV subunitvaccine, an attempt was made to find a new way to prepare IBD vaccine containing glycosylated mVP2antigen. Firstly, IBDV mVP2 gene (with a nucleic acid sequence encoding B cell epitope of IBDV(...
متن کاملRec. ST6Gal-I variants to control enzymatic activity in processes of in vitro glycoengineering
Background Glycosylation is an important posttranslational modification of proteins influencing protein folding, stability and regulation of the biological activity. The sialyl mojety (sialic acid, 5-N-acetylneuramic acid) is usually exposed at the terminal position of N-glycosylation and therefore, a major contributor to biological recognition and ligand function, e.g. IgG featuring terminal s...
متن کاملexpression, purification and glycosylation analysis of chicken infectious bursal disease virus vp2 in yeast
infectious bursal disease (ibd), a highly contagious and devastating disease in young chicken, is causedby infectious bursal disease virus (ibdv). to improve the immunogenicity of recombinant ibdv subunitvaccine, an attempt was made to find a new way to prepare ibd vaccine containing glycosylated mvp2antigen. firstly, ibdv mvp2 gene (with a nucleic acid sequence encoding b cell epitope of ibdv(...
متن کاملGlycoengineering of yeasts from the perspective of glycosylation efficiency.
N-linked glycosylation of proteins is one of the most common posttranslational modifications. N-glycan structures and N-glycosylation efficiency are crucial parameters in the production of N-glycosylated proteins. Yeast cells can be seen as an attractive production host for therapeutic glycoproteins and pioneering work of glycoengineering was performed in Pichia pastoris, realizing yeast strain...
متن کاملthe effect of some flavonoids on in-vitro non-enzymatic glycosylation of proteins
introduction: non-enzymatic glycosylation of proteins which is the major cause of diabetic complications, cardiovascular diseases retinopathy, nephropathy and neuropathy can effectively be inhibted by antioxidants. materials and method: the antioxidant effects of several flavonoids, such as rutin, kaempferol, quercetin, apigenin, naringin, morin and biochanin a were studied on glycosylation of...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2018
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.1718172115